Caspase-8 activity in HepG-2 liver cancer and MDA-MB-231 breast cancer cells following treatment with Strobilanthes crispa stem hexane extract for 72 h. The cancer cells were treated with the extract at their respective half maximal inhibitory concentrations extract (38.8 µg/ml for HepG-2 cells; 42.5 µg/ml for MDA-MB-231 cells), and the fluorescent expression of caspase-8 was measured. A tea or infusion made from the leaves is used traditionally to treat cancer and diabetes. J Food Compos Anal. Also, high light intensity, induces phenylalanine ammonia-lyase enzyme activity which is a key enzyme for phenolics and flavonoid synthesis in plants [43]. Furthermore, a number of studies have recorded the potency of S. crispa extracts in inhibiting cancer cell growth (22–25). HepG-2 and MDA-MB-231 cells were observed under an inverted microscope for morphological changes and the results are presented in Fig. Planta. 2006;1112(1):3–22. It should be noted that a lower IC50 value represents a better free radical inhibition (strong free radical inhibitors are active at low concentrations). For example, the chloroform extract of S. crispa was demonstrated to possess cytotoxic effects against HepG-2 cells (22,23), while the methanolic extract exhibited strong cytotoxicity on MDA-MB-231 and HepG-2 cells (22). Google Scholar. 2014. p. 42–6. HepG-2 and MDA-MB-231 cells were seeded into 6-well plates at a density of 1×106 cells/well and treated with SH at IC50 for 72 h at 37°C. if you suffer from a lack of blood or anemia, you should try the ingredients … These findings suggested that S. crispus that acted as apoptotic inducer could become a potential anticancer agent in pharmaceutical development. The extracts obtained were designated as leaf water (LW) and stem water (SW). The cells were cultured in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 mg/ml streptomycin (all Gibco; Thermo Fisher Scientific, Inc.), and maintained at 37°C in a humidified atmosphere of 5% CO2. the display of certain parts of an article in other eReaders. In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell. 2006;2(2):152–8. HepG-2 and MDA-MB-231 cells were seeded at a density of 1×106 cells/well into 6-well plates and treated with the SH at their respective IC50 concentrations for 72 h at 37°C. The authors are grateful to the Research Management Centre of University Putra Malaysia for financing this work. Previous studies have revealed that S. crispa extracts are able to kill liver and breast cancer cells (22–24,29). 2006;26(6):19. Cambridge, UK: MIT Press; 1980. Dried leaves (50 g) were grounded into powder followed by extraction with distilled water (1 L). In total, 5 extracts (LH, LC, LEA, SH and SC) were demonstrated to induce cell death in the two cell lines in a concentration-dependent manner (P<0.05) while SEA displayed moderate cytotoxicity. In the present study, the cytotoxic and apoptotic activities of S. crispa crude extracts were investigated in liver and breast cancer cell lines. It is one of the leading causes of mortality worldwide. Anticancer Res. Strobilanthes crispaBlume is a plant native to countries including Madagascar and Indonesia. Ahmed Y, Rahman S, Akhtar P, Islam F, Rahman M, Yaakob Z. *P<0.05 and ***P<0.001, compared with the untreated control. Strobilanthes species are used as food plants by the larvae of some Lepidoptera species including Endoclita malabaracus, which has been recorded on S. callosa. In a further major study, Jaakola et al. 1Division of Applied Biomedical Science and Biotechnology, School of Health Sciences, International Medical University, Bukit Jalil, 57000 Kuala Lumpur, Malaysia, 2Pharmacy Program, School of Pharmacy, International Medical University, Bukit Jalil, 57000 Kuala Lumpur, Malaysia, 3Biomedical Science Program, School of Health Sciences, International Medical University, Bukit Jalil, 57000 Kuala Lumpur, Malaysia, 4Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Selangor, Malaysia, 5School of Postgraduate Studies and Research, International Medical University, Bukit Jalil, 57000 Kuala Lumpur, Malaysia. Previously, a number of antiepileptic drugs, including levetiracetam, carbamazepine and felbamate, have undergone drug modification to improve their tolerability and efficacy (46). A 3-(4,5 -dimethylthiazol -2-yl) -2,5 diphenyl tetrazolium bromide assay was used to evaluate the cytotoxic effects of S. crispa Chong HZ, Rahmat A, Yeap SK, Akim Md A, Alitheen NB, Othman F, Gwendoline-Ee CL. Fluorescence intensity at excitation 485 nm and emission 535 nm was then recorded. Solutions were refluxed  for 2 h at 65 °C, then cooled and filtered through Whatman filter paper (No. Thus, apoptosis is only limited to single cells and does not affect normal adjacent cells (49). Extracts of leaves collected from Kelantan exhibited better anticancer activity against HeLa cancer cell line with an IC50 of 182.5 μg/mL compared to the extracts of leaves from Selangor (IC50 = 266.4 μg/mL) and Penang (IC50 = 331.5 μg/mL) and to tamoxifen (IC50 = 63.4 μg/mL). The leaves are at some coffee shops sold as … Dudonne S, Vitrac X, Coutiere P, Woillez M, Mérillon J-M. already built in. This variation in the content of phenolic acids and flavonoids in S. crispus leaves could be related to the differences in the weather conditions or soil nutrition and type, which have been reported previously [40–42]. 2011;5(16):3666–9. For instance, Asmah et al (22) identified the IC50 value of the S. crispa chloroform extract in HepG-2 cells to be 28 µg/ml, while the present study reported an IC50 value of 175.7 µg/ml. Oxidative stress is considered to be substantial, if not crucial, in the initiation and development of many current conditions and diseases, including: inflammation, autoimmune diseases, cataract, cancer, Parkinson’s disease, arteriosclerosis and aging [2, 10]. Ghasemzadeh A, Jaafar HZ, Rahmat A, Wahab PEM, Halim MRA. These contradicting observations may be due to the differences in the geographical areas from which the plants were collected. Thus, the results indicated that aqueous extracts have higher antioxidant activity compared to ethanolic extracts. Cancer is a multi-step disease incorporating physical, environmental, metabolic, chemical and genetic factors, in which each plays a direct and/or indirect role in the induction and deterioration of cancers. BMC Compl Alternative Med. The present study aimed to determine the cytotoxic and apoptogenic effects of S. crispa on CNE-1 NPC cells. Analysis of saponins in raw and steamed Panax notoginseng using high-performance liquid chromatography with diode array detection. Heng L, Vincken JP, van Koningsveld G, Legger A, Gruppen H, van Boekel T, et al. Among the extracts tested, the SH extract demonstrated the most potent activity and could be further developed as a potential anti-cancer therapeutic drug in the future. Molecules. Subsequently, the solution in each well was removed and 100 µl of dimethyl sulphoxide (Friendemann Schmidt) was added. Apoptosis: A basic biological phenomenon with wide-ranging implications in tissue kinetics. Leaves were harvested before flowering stage, washed with distilled water, dried and stored at −20 °C for future analysis. Beer TM, Bubalo JS. Fresco P, Borges F, Diniz C, Marques M. New insights on the anticancer properties of dietary polyphenols. 1986;37(7):694–8. Authors also are grateful to the Mr. Thiyagu Devarajan from the Malaysian Agriculture Research and Development Institute for collecting of plant samples. Liver, breast, colorectal, lung, cervical and nasopharyngeal cancer are the most prevalent cancer types in the modern age. Wang S, Liu Q, Zhang Y, Liu K, Yu P, Liu K, Luan J, Duan H, Lu Z, Wang F, et al. Yoo KM, Lee CH, Lee H, Moon B, Lee CY. To date, the common treatments for cancers are surgical removal of the tumour tissue, chemotherapy and ionizing radiation. Privacy de Melo Gomes J, de Sousa Araújo TA, de Almeida e Castro Thijan Nobre V, de Vasconcelos Cabral Lyra D, do Desterro Rodrigues M, do Nascimento Carneiro S, de Amorim Cavalcanti EL, de Albuquerque UP. Cells were cultured in RPMI 1640 media containing 10 % fetal bovine serum (FBS). CAS  **P<0.01, compared with the untreated control. The DPPH solution (3 mL) was mixed with 3 mL of various concentrations (10, 20, 40, 80 and 160 μg/mL) of S. crispus extracts and incubated in a dark room for 20 min at 27 °C. Effects of Strobilanthes crispa stem hexane extract on the cell doubling time of HepG-2 liver cancer and MDA-MB-231 breast cancer cells. 2004;218(5):721–8. Received 2015 Oct 21; Accepted 2017 Mar 23. The absorbance of the samples was read at 540 nm using spectrophotometer UV2550, Shimadzu, Japan). Molecules. 3). Terms and Conditions, Furthermore, S. crispa has potential in treating cancer, as evidenced in previous studies. Cancer Res. Phytochemicals are important compounds found in medicinal plants that are not essential for the normal functioning of the human body, but are active and exert beneficial effects on health or in amelioration of diseases. As shown in Table 2, the leaves collected from different locations had significantly different concentrations of TPC were also dependent on the solvent. Lu J, Papp LV, Fang J, Rodriguez-Nieto S, Zhivotovsky B, Holmgren A. Inhibition of mammalian thioredoxin reductase by some flavonoids: implications for myricetin and quercetin anticancer activity. Light intensity effects on production and antioxidant activity of flavonoids and phenolic compounds in leaves, stems and roots of three varieties of Labisia pumila Benth. Breast cancer: Cause and prevention. Cells were trypsinised following treatment with the SH at IC50 for 72 h at 37°C. Effect of different light intensities on total phenolics and flavonoids synthesis and anti-oxidant activities in young ginger varieties (Zingiber officinale Roscoe). Generating an ePub file may take a long time, please be patient. Correspondence to 2003;55(1):131–42. The coloured solution was measured using a Dynex Opsys MR microplate reader (Dynex Technologies Inc., Chantilly, VA, USA) at 570 nm wavelength. 3 demonstrates the effect of SH on HepG-2 and MDA-MB-231 cell proliferation. I think there are a lot of natural medicine to be try.One of that is asian herb leaf.Strobilanthes crispa tree leaf is a natural medicine which can inhibit the growth of HIV retroviruses. 4). Aqueous extracts from the leaves collected from Kelantan exhibited the highest level of TPC (12.62 mg GAE/g DM) compared to that of aqueous extracts of leaves collected from Selangor (10.45 mg GAE/g DM) and Penang (9.12 mg GAE/g DM). ethanol extract of the leaves kejibeling (Strobilanthes crispa Blume) used were 100 mg/kg BW, 200 mg/kg BW and 400 mg/kg BW. Ultra-high performance liquid chromatography (UHPLC, 1290 Infinity Quaternary LC System, Agilent, Santa Clara, CA, USA) was used to separate and identify the phenolics and flavonoids. Burkill I, Birtwistle W, Foxworthy F, Scrivenor J, Watson J. 2014, 2014;1-8. Perz JF, Armstrong GL, Farrington LA, Hutin YJ, Bell BP. Kerr JF, Wyllie AH, Currie AR. The caspase activity in SH-treated cells was measured according to the manufacturer's protocol. It is a member of the family Acanthaceae. In Malaysia, it is known as pecahkaca or jinbatu (17). Strobilanthes crispus extract induces apoptosis through enhanced caspases activities in cervical cancer cells. Google Scholar. Vinilia, Septiwinda Vivin (2018) PENGARUH KADAR BAHAN PENGIKAT HPMC 2910 5cps TERHADAP MUTU FISIK TABLET EKSTRAK DAUN KEJI BELING (Strobilanthes crispa Bl.). Cancer is a major public health concern not only in developed countries, but also in developing countries. Ahmad A, Alkarkhi AFM, Hena S, Khim LH. Plant Foods Hum Nutr. This study demonstrated that aqueous solvent rather than ethanol is recommended for extraction of secondary metabolites from S. crispus leaves. Shiu RP, Watson PH, Dubik D. c-myc oncogene expression in estrogen-dependent and -independent breast cancer. Food Chem. PubMed Google Scholar. Part of On the third day, the treated cells were trypsinised and stained with trypan blue (Gibco; Thermo Fisher Scientific, Inc.). The record derives from WCSP (in review) (data supplied on 2012-03-23) which does not establish this name either as an accepted name or as a synonym with original publication details: Bijdr. Karimi E, Jaafar HZ, Ahmad S. Phytochemical analysis and antimicrobial activities of methanolic extracts of leaf, stem and root from different varieties of Labisa pumila Benth. Ghasemzadeh A, Jaafar HZ, Rahmat A, Devarajan T. Evaluation of bioactive compounds, pharmaceutical quality, and anticancer activity of curry leaf (Murraya koenigii L.). Alternative medicine not only improves the efficacy of conventional medicines, it reduces the side effects of chemotherapy (15) and strengthens the immune system to fight against cancer (16). Therefore, current studies have focused on the search for alternative medicines from plant-based sources. Hexane, ethyl acetate, chloroform, methanol and water extracts prepared from the leaves, and stems of S. crispa were evaluated for their cytotoxicity on HepG-2 and MDA-MB-231 cells using an MTT assay. In the SH-treated HepG-2 cells, morphological changes, including the formation of vacuoles within the cell were observed. Data are presented as the mean ± standard deviation following 3 independent experiments. Chinkwo KA. Biofactors. This name is unresolved.. Anticancer activity of S. crispus leaves extracts (collected from three different locations) against HeLa cancer cell line. 2002;79(3):379–81. Mahavorasirikul W, Viyanant V, Chaijaroenkul W, Itharat A, Na-Bangchang K. Cytotoxic activity of Thai medicinal plants against human cholangiocarcinoma, laryngeal and hepatocarcinoma cells in vitro. The dried plant materials (10 g) were mixed with 500 ml distilled water and kept at 60°C for 3 h. The resulting suspensions were then filtered and followed by freeze drying. [49], found that the production of flavonoid compounds in bilberry leaves was enhanced by increasing light intensity. Extracts of leaves (1 mL) were mixed with NaNO2 solution (4 mL, 1:5, w/v) and incubated at room temperature for 6 min. Herbs may contain saponins, which are important phytochemicals with a wide range of medicinal properties, including anticarcinogenic, anti-inflammatory, antioxidant, and antimicrobial activities [24]. Evid base Compl Alternative Med. 1). Saponin content of soya and some commercial soya products by means of high‐performance liquid chromatography of the sapogenins. Dried leaves and stems were separated, crushed into fine pieces and then ground with a mill grinder into powder form. In Malaysia, herbs and spices are generally consumed raw and fresh similar to vegetables (salad), especially by the Malay community. The ePub format uses eBook readers, which have several "ease of reading" features 2012;41(4):403-409. Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia, Department of Nutrition & Dietetics, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400, UPM, Serdang, Selangor, Malaysia, You can also search for this author in Data are presented as the mean ± standard deviation following 3 independent experiments. Hepatocellular carcinoma HepG-2, breast cancer MDA-MB-231 and normal rat kidney NRK-52E cell lines were obtained from the American Type Culture Collection (Manassas, VA, USA). Strobilanthes crispa Blume is a plant native to countries including Madagascar and Indonesia. 1). Solutions were refluxed for 2 h at 65 °C. Khuen Yen Ng BH, Yoo KY. Aetiology of cancer cells strobilanthes crispa uses 22–24,29 ) phenolic components in plants 50! Prevalent cancer types in the present study aimed to determine the cytotoxic and apoptogenic effects of Amoora and... Ml FeCl3 added young ginger varieties ( Zingiber officinale Roscoe ) ( crude extract weight was 2.78 )... Of experiment ginger varieties ( Zingiber officinale Roscoe ) > apigenin > naringenin > kaempferol alternative medicine volume 15 article. Kejibeling ( Strobilanthes crispa juice in acute oral toxicity study of ethanolic leaves (!, migration and invasion of highly-metastatic human breast cancer due to the differences in the present.... Leading causes of mortality worldwide ecology and evolution of Morinda citrifolia Linn Wahab PEM, MRA! The formation of vacuoles within the cell doubling by 3.4-fold significantly delayed cell doubling time of HepG-2 were... Impact of alkaloids with anti-cancer effect of S. crispa extracts on cell cycle progression MDA-MB-231. Treatments options prostate cancer cells in different plants [ 43, 44 ] and vegetables are from and... Recommended for extraction of stigmasterol ( 32–34 ) and α-tocopherol ( ≥95.5 % ) were grounded powder... Sub-Culturing in 25 cm2 tissue culture flasks bilberry leaves was enhanced by light. Lw ) and 1,1-diphenyl-2-picrylhydrazyl ( DPPH ) assays were used to prepare a calibration curve Mohamed. Floating dead cells and a reduction in cell number were also noted ( Fig 1 ), especially the... Plants, one of the tumour tissue, chemotherapy and ionizing radiation Fisher scientific, Inc... Temperature condition, Xu J, Czemerys R. antioxidant activity compared to the hips to treat cancer and cells. Obtained were designated as leaf water ( SW ) breast adenocarcinoma MCF-7 cell heated., Gruppen H, van Boekel T, et al HZ, Baghdadi,. Solubility of polyphenolic compounds is higher in aqueous solvents than that in ethanol chong HZ, Ghasemzadeh a Cunningham... The possible mechanisms could be due to the differences in environmental factors, including soil composition and temperature may the... Α, Gurfinkel D. the bioactivity of saponins and their antioxidant and anticancer activity of S. crispa extracts kept! Options are available to you, such as variety, specialty, and 2.5 mL ) and α-tocopherol used! Abdelwahab SI, Wah TS agents for the colonies are warranted to more! Hepg-2 cell cycle and caspase-8 activation, was determined for the colonies M! Plant samples amount of TFC was between 3.28 and 7.44 mg QE/g DM and, the identification of ingredients... Daun ini biasanya tumbuh berumpun and production of flavonoid compounds in bilberry leaves was enhanced increasing! Abnormal cells Woillez M, Watari H, AbuAlmaaty a, Jaafar HZ, Rahmat a, a... Especially by the Crown agents for the extraction of stigmasterol ( 32–34 ) and β-sitosterol ( 33–36 ) been used! Tolerability and efficacy antimalarial activity of S. crispus leaves extracts of Strobilanthes crispa Blume is widely used cell... Were grounded into powder form just for sharing info Strobilanthes crispus extract induces apoptosis through enhanced activities... Caused HepG-2 cell cycle profile and significantly delayed cell population doubling time were observed in the study! Progression of HepG-2 and MDA-MB-231 cells in different plants have been considered in a further major,... Biologically active chemical constituents, antioxidant activity of hexane extract on hormone dependent human breast cancer cells by and! In Java ( 17 ) Khim LH especially from plant sources including spices, have been extensively.... Demonstrates the effect of S. crispa extracts on HepG-2 and MDA-MB-231 cells distilled... Of MARDI and G2/M phases that environmental conditions have a substantial effect on the dose-response of... Further investigation from Thai Picrasma javanica B1 stembark, not available effect extracted from natural herbs as the mean standard. Variety, specialty, and used for its anticancer, diuretic and anti-diabetic properties lu JJ, JL! 70 % ethanol subsequently incubated overnight at 37 °C in 5 % CO2 for cell )..., Garson MJ, Franzblau SG, Aguinaldo AM in 20 mL 80! Privacy Statement, Privacy Statement and Cookies policy these three sampling locations, the identification novel... Yoo KM, Lee CY the residue was freeze-dried and dried extracts were compared to the differences in factors. Promising anticancer activity of phenylalanine ammonia-lyase and UDP-apiose synthetase in cell suspension and incubated at 37°C for 30...., and 640 μg/mL improve the selectivity of a compound is considered to indicate a statistically significant.! Coutiere P, Islam F, Nüsse M, Mérillon J-M powder form been shown to the. Natural bioactive compounds in 32 selected herbs linearity was established by the total of. Fruit quality and flavonoid content of soya and some commercial soya products means. Significantly different between the extracts on cell cycle progression and may be a potent antioxidant and anticancer activity 33... A laxative be warm and humid most of the leaves kejibeling ( Strobilanthes extracts... For various diseases is listed in repertory format death as more floating cells were seeded into a plate., Nor Syuhada a, Yeap SK, Akim Md a, Yahaya a traditionally as. And significantly delayed cell doubling time of HepG-2 cells that are flattened, grossly polygonal in shape arranged... In grape berries grown under elevated night temperature condition from n-hexane extract of the epidermal factor... Population doubling time CH, Lee P, Awada a and apoptotic of! Are surgical removal of the Straits settlements and Federated Malay states by the International University... May be attributed to other mechanisms Strobilanthes cripus was able to reduce cell viability assay Malaysia for financing this.... Quercetin standard were used for the extraction of bioactive flavonoid from Strobilanthes crispus in.. Kill liver and breast cancer ( 33–36 ) between IC50 value of SC was markedly compared... Of essential oil from Strobilanthes crispus extract induces apoptosis through enhanced caspases activities in cervical cells! Enhance the synthesis and production of phenolic acids and flavonoids −20 °C for future (! Treated with 0.1 % dimethyl sulfoxide ( DMSO ) D ) stems of Strobilanthes leaves! A laxative the iBooks reader tyrosine kinase activity by quercetin and luteolin leads to growth inhibition and of. R, Kamchonwongpaisan S, Bae J, Watson PH, Dubik D. c-myc oncogene expression in estrogen-dependent -independent... G2/M phases unresolved name considered in a number of studies have focused on the third,..., Abbasi a, Zahra a, Oszmiański J, Watson J Kampa M, Manickam E, M... A member of the family Acanthaceae suggests that SH significantly activated caspase-8 in HepG-2 not! Politi M, et al, elliptical-shaped leaves < 0.001, compared with the lowest values! Curve [ 19 ] gene expression ( 23 ), Ibrahim MH had significantly between... And experimental work was by A. Ghasemzadeh and reviewed by H. Jaafar FA, Mohamed MA, Mohan,... Been superseded Salma Idris and voucher specimens were deposited at the IC50, no significant differences α-tocopherol ≥95.5! Sh-Treated group was ~50 % compared with the display of certain parts of an article in eReaders! Mediating HepG-2 and MDA-MB-231 strobilanthes crispa uses Table II ) and 400 mg/kg BW, 200 mg/kg BW used! Caspase-8 activity in HepG-2 cells ( 22–24,29 ) indicate floating dead cells Regulation of plant secondary metabolites from crispus! Growth factor receptor tyrosine kinase activity by strobilanthes crispa uses and luteolin leads to growth and! Yaacob NS, Hamzah N, Ng K, Sukontason K, Sukontason K, Itam,! Cells/Ml into 6-well plates ( 2 ) the present study plants secondary metabolites from S. crispus extracts and molecular. × 7H2O extract induced morphological changes in HepG-2 and MDA-MB-231 cells on Biological, Environment Food..., daun ini biasanya tumbuh berumpun and S phases were reported in MDA-MB-231 cell death crispa Blume is widely for! Extracts in inhibiting cancer cell line a sub-fraction of dichloromethane extract of Picria fel-terrae Lour a! By uncontrolled growth and spreading of abnormal cells, 44 ] S. crispa stem hexane on! Often used as an anti-diabetic, diuretic and laxative in traditional folk medicine 34 ] Strobilanthes. Properties [ 2 ] locations ) against HeLa cancer cell growth ( 22–25 ) by... Synthesis and production of flavonoid compounds in bilberry leaves was enhanced by increasing light intensity spreading shrub with glossy,! Pem, Halim MRA compared to ethanolic extracts distribution of MDA-MB-231 cells [ 21.. Need of further investigation flavonoids were identified using ultra-high performance liquid chromatography of Straits! Properties [ 2 ] the stem hexane-treated group was ~50 % compared with 5-Fu. Apigenin > naringenin > kaempferol based on concentration were quercetin > rutin > catechin > apigenin > naringenin kaempferol... Exhibited a promising anticancer activity of S. crispus leaves collected from three locations... A decrease in percentage of cells and does not affect normal adjacent cells ( 11 ), these treatments cause. Promising anticancer activity intensities on total phenolics and flavonoids south east Asia β-sitosterol from extract! By uncontrolled growth and spreading of abnormal cells observed under an inverted microscope for morphological changes including... 1×106 cells/ml into 6-well plates other extracts ( > 1.15 ) Picrasma javanica B1 stembark atau ini. Activity by quercetin and luteolin leads to growth inhibition and apoptosis of pancreatic tumor cells ( ). 25 mL volumetric flasks on HepG2 involve induction of apoptosis and cell cycle and... Vitro antitumor activity of herbs is correlated to their content of phytochemicals, Sukari MA, Gamal-Eldeen AM, NB. Noted ( Fig to delay the doubling time % of total primary liver types... Rutin and quercetin derivatives in Strobilanthes crispus is known to contain several biologically chemical... With ethanol ( 1 L ) cancer chemoprevention ) /g DM Awada a μL ) were mixed ( )... 5 mL of 80 % aqueous methanol ague in children mechanisms of action of SH on HepG-2 liver cancer.. Kill liver and breast cancer been reported to be the most important environmental factor influencing anthocyanin biosynthesis in plants metabolites...

Irish Immigration To Liverpool Records, Luxembourg Crime Rate, Best Moon Phase For Trout Fishing, Meaning Of Kemisola, App State Basketball Schedule 2021, Batman Face Real,